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Aluminium Oxide Chromatography

Aluminium Oxide Chromatography

Aluminum Oxide is one of the strong adsorbent media, which is highly preferred in column chromatography
techniques. Active aluminum oxide is a typical amphoteric (acting either as a base or an acid and being
configured as neutral ) that provides the best results for a chromatographer to separate many compounds over and
above silica gels. Alumina can act as a weak ion exchanger demonstrating anionic or cationic properties while
acting as an adsorbent. Alumina, due to its unique biological characteristics, is a special adsorbent for use in
separation sciences. These active media are available in three different grades as per their application.

  • Acidic
  • Basic
  • Neutral

This active media is a white to fine off-white particles, which are highly porous. Available grades are very
effectively sensitive to aromatic compounds. Mostly Alumina acidic works and provides useful separation with weak
acids where these can be improved by using basic solvents.
Acidic & Basic alumina Oxide is used to do the separation of sensitive material.

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Application

  • Isolation & purification of antibiotics
  • Dehydration of organic solvents
  • Enzyme purification
  • Isolation of glycosides
  • Removal of peroxides from organic solvents
  • Purification of organic solvents.
  • Purification of natural products & herbals like reserpine, capsaicin and oleoresin.
  • Purification of dyes, dye intermediates, alkaloids, steroids, liquids, amino acids, hormones, aliphatic & aromatic hydrocarbons and vitamins.
Aluminium Oxide Chromatography

Technical Data Sheet

Grades Alumina-Basic Alumina-Neutral Alumina-Acidic
pH (5% Aqueous Solution 9.5 ± 0.5 7.0 ± 0.5 4.5 ± 0.5
Specific Surface [m2/gm] 150 ± 10% 150 ± 10% 150 ± 10%
Brockman Activity I -II I -II I -II
Mean Pore Diameter [A] 60 60 60
Pore Volume [ml/gm] ~ 0.25 ~ 0.25 ~ 0.25
CI [mval/g] Nil 0.03 0.14
Fe2O3 [%] 0.01 – 0.03 0.01 – 0.03 0.01 – 0.03
Na2O [%] 0.25 – 0.4 0.25 – 0.4 0.25 – 0.4
Bulk Density ~ 920 ~ 920 ~ 920
Remarks: All the parameters to be customized as per the client requirement.
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